On Feb. 5th, 2015, Prof. Jixin Chen from Department of chemistry and biochemistry, Ohio University visited SINANO and delivered a talk entitled “Super-Resolution Fluorescence Imaging for Single-Molecule Dynamics at Interfaces”. The presentation was chaired by Prof. Liwei Chen.
Super-resolution optical microscopy/nanoscopy has greatly extended our understanding of many systems, including living cell structures and dynamics, and catalytically hot-spots on a nanoparticle. Super-resolution techniques allow the capture of images with a higher resolution (<20 nm) than the diffraction limit, ~250 nm for a typical fluorescence image.
In this talk, Dr. Chen first introduced the principles of a few super-resolution optical microscopies, especially the single-molecule localization method. Then he shared his experience in the application of a super-resolution imaging technique in the measurement of single protein-ligand interaction. This research was motivated by the pharmaceutical industry for protein separation with ion-exchange chromatography. Then he also shared his work in single DNA localization. Thorough discussion on the impressive results was carried out after presentation.